transwell boyden chambers Search Results


90
Corning Life Sciences 24-well transwell boyden chamber consisting of inserts membranes
24 Well Transwell Boyden Chamber Consisting Of Inserts Membranes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/24+well+transwell+boyden+chamber+consisting+of+inserts+membranes/pm36290354-59-19-26
Average 90 stars, based on 1 article reviews
24-well transwell boyden chamber consisting of inserts membranes - by Bioz Stars, 2026-09
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90
Corning Life Sciences transwell boyden chamber
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Transwell Boyden Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/transwell+boyden+chamber/pmc05797023-130-12-18
Average 90 stars, based on 1 article reviews
transwell boyden chamber - by Bioz Stars, 2026-09
90/100 stars
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90
Corning Life Sciences modified boyden chamber plate with 8-μm pore size polycarbonate membrane filters
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Modified Boyden Chamber Plate With 8 μm Pore Size Polycarbonate Membrane Filters, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/modified+boyden+chamber+transwell+polycarbonate+filters/pmc07905040-80-8-18
Average 90 stars, based on 1 article reviews
modified boyden chamber plate with 8-μm pore size polycarbonate membrane filters - by Bioz Stars, 2026-09
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90
Corning Life Sciences modified boyden chambers transwells corning/costar no. 3422
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Modified Boyden Chambers Transwells Corning/Costar No. 3422, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/modified+boyden+chambers+transwells+corning+costar++3422/pmc03834344-81-7-10
Average 90 stars, based on 1 article reviews
modified boyden chambers transwells corning/costar no. 3422 - by Bioz Stars, 2026-09
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90
Corning Life Sciences boyden chambers transwell membrane filter inserts
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Boyden Chambers Transwell Membrane Filter Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/boyden+chambers+transwell+membrane+filter+inserts/pm36561964-83-7-17
Average 90 stars, based on 1 article reviews
boyden chambers transwell membrane filter inserts - by Bioz Stars, 2026-09
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90
Becton Dickinson boyden chamber transwell system
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Boyden Chamber Transwell System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/transwell+boyden+chamber/pmc05553295-106-15-23
Average 90 stars, based on 1 article reviews
boyden chamber transwell system - by Bioz Stars, 2026-09
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90
Corning Life Sciences 24-well transwell boyden chamber covered by a aperture polycarbonate membrane
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
24 Well Transwell Boyden Chamber Covered By A Aperture Polycarbonate Membrane, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/24+well+transwell+boyden+chamber+covered+by+a+aperture+polycarbonate+membrane/pmc09273941-131-15-22
Average 90 stars, based on 1 article reviews
24-well transwell boyden chamber covered by a aperture polycarbonate membrane - by Bioz Stars, 2026-09
90/100 stars
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90
Corning Life Sciences 24-well boyden chamber costar transwell inserts with a 8-μm pore size
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
24 Well Boyden Chamber Costar Transwell Inserts With A 8 μm Pore Size, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/24+well+boyden+chamber+costar+transwell+inserts+with+a+8+%CE%BCm+pore+size/pmc07719785-113-17-19
Average 90 stars, based on 1 article reviews
24-well boyden chamber costar transwell inserts with a 8-μm pore size - by Bioz Stars, 2026-09
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90
Becton Dickinson transwell 24-well boyden chambers
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Transwell 24 Well Boyden Chambers, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/transwell+24+well+boyden+chambers/10__1039_slash_c9qm00044e-112-9-14
Average 90 stars, based on 1 article reviews
transwell 24-well boyden chambers - by Bioz Stars, 2026-09
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90
Corning Life Sciences boyden chambers polycarbonate filters
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Boyden Chambers Polycarbonate Filters, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/transwell+boyden+chambers+with+polycarbonate+filter+of+pore+size/10__1111_slash_j__0022___202x__2005__23881__x-109-8-13
Average 90 stars, based on 1 article reviews
boyden chambers polycarbonate filters - by Bioz Stars, 2026-09
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90
Corning Life Sciences boyden chamber (transwell) assay pore-size membrane inserts
( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) <t>Transwell</t> analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Boyden Chamber (Transwell) Assay Pore Size Membrane Inserts, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/boyden+chamber++transwell++assay+pore+size+membrane+inserts/ppr0777367-45-26-29
Average 90 stars, based on 1 article reviews
boyden chamber (transwell) assay pore-size membrane inserts - by Bioz Stars, 2026-09
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90
Becton Dickinson boyden transwell chamber 8-μm pore size
IL-1β increases the metastatic potential of melanoma cancer cells. ( A , B ) B16F10 cells were plated in upper chamber of <t>transwell.</t> Serum-free medium containing recombinant IL-1β (0.5, 1 ng/ml) was placed in the bottom chamber. After 24 hr, the migration or invasion of B16F10 cells was determined. Values are means ± SEM (n = 3). * p < 0.05.
Boyden Transwell Chamber 8 μm Pore Size, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transwell+boyden+chambers/boyden+transwell+chamber+8+%CE%BCm+pore+size/pmc06706417-180-6-12
Average 90 stars, based on 1 article reviews
boyden transwell chamber 8-μm pore size - by Bioz Stars, 2026-09
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Image Search Results


( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) Transwell analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).

Journal: Oncotarget

Article Title: miR-30a suppresses lung cancer progression by targeting SIRT1

doi: 10.18632/oncotarget.23529

Figure Lengend Snippet: ( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) Transwell analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).

Article Snippet: The invasion ability and apoptosis of A549 cells was tested in a Transwell Boyden Chamber (6.5 mm, Costar, Corning, NY, USA) and Annexin V-FITC/PI staining kit (BD Biosciences, San Diego, CA, USA).

Techniques: Proliferation Assay, Transfection, Negative Control, Control, Plasmid Preparation, Over Expression, Apoptosis Assay

IL-1β increases the metastatic potential of melanoma cancer cells. ( A , B ) B16F10 cells were plated in upper chamber of transwell. Serum-free medium containing recombinant IL-1β (0.5, 1 ng/ml) was placed in the bottom chamber. After 24 hr, the migration or invasion of B16F10 cells was determined. Values are means ± SEM (n = 3). * p < 0.05.

Journal: Scientific Reports

Article Title: Inhibition of NLRP3 inflammasome in tumor microenvironment leads to suppression of metastatic potential of cancer cells

doi: 10.1038/s41598-019-48794-x

Figure Lengend Snippet: IL-1β increases the metastatic potential of melanoma cancer cells. ( A , B ) B16F10 cells were plated in upper chamber of transwell. Serum-free medium containing recombinant IL-1β (0.5, 1 ng/ml) was placed in the bottom chamber. After 24 hr, the migration or invasion of B16F10 cells was determined. Values are means ± SEM (n = 3). * p < 0.05.

Article Snippet: Cell invasion assay were performed using Boyden transwell chamber (8-μm pore size, Becton Dickinson).

Techniques: Recombinant, Migration