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Image Search Results
Journal: Oncotarget
Article Title: miR-30a suppresses lung cancer progression by targeting SIRT1
doi: 10.18632/oncotarget.23529
Figure Lengend Snippet: ( A ) A cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-30a mimic or scrambled negative control RNA. ( B ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the miR-3a inhibitor or scrambled negative control RNA. ( C ) The cell proliferation assay was performed 12, 24, 36 and 48 hours after the transfection of A549 cells with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. ( D–F ) The apoptosis assay was performed 24 hours after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. (D and E) representative image; (F) quantitative analysis. ( G and H ) Transwell analysis was performed after the transfection of A549 cells with equal doses of the miR-30a mimic, miR-30a inhibitor or scrambled negative control RNA or with equal doses of the pre-miR-control plus control plasmid, pre-miR-control plus SIRT1 overexpression plasmid, miR-30a mimic plus control plasmid, or miR-30a mimic plus SIRT1 overexpression plasmid. G. representative image; H. quantitative analysis. The results are presented as the mean ± S.E. of three independent experience ( * p < 0.05; ** p < 0.01; *** p < 0.005).
Article Snippet: The invasion ability and apoptosis of A549 cells was tested in a
Techniques: Proliferation Assay, Transfection, Negative Control, Control, Plasmid Preparation, Over Expression, Apoptosis Assay
Journal: Scientific Reports
Article Title: Inhibition of NLRP3 inflammasome in tumor microenvironment leads to suppression of metastatic potential of cancer cells
doi: 10.1038/s41598-019-48794-x
Figure Lengend Snippet: IL-1β increases the metastatic potential of melanoma cancer cells. ( A , B ) B16F10 cells were plated in upper chamber of transwell. Serum-free medium containing recombinant IL-1β (0.5, 1 ng/ml) was placed in the bottom chamber. After 24 hr, the migration or invasion of B16F10 cells was determined. Values are means ± SEM (n = 3). * p < 0.05.
Article Snippet: Cell invasion assay were performed using
Techniques: Recombinant, Migration